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. 2020 Dec 30;22(1):300. doi: 10.3390/ijms22010300

Figure 4.

Figure 4

Effect of bFGF on the chondrogenesis of SMSCs in 3D chondrogenic culture: histological evaluation. Synovial pellets from both the bFGF (+) and bFGF (−) groups cultured in chondrogenic medium (without bFGF) for 4 weeks. (a) Images of Saf-O staining and immunostaining for COL2 (patients #1 to #5) (bar = 500 µm). (b) High-magnification images of Saf-O, Arcian Blue, Sirius Red, and COL2 staining (patient #4) (bar = 50 µm). bFGF, basic fibroblast growth factor; SMSCs, synovial mesenchymal stem cells; 3D, three-dimensional; Saf-O, Safranin-O; COL2, collagen type II.