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. 2020 Dec 24;22(1):97. doi: 10.3390/ijms22010097

Figure 1.

Figure 1

Effect of Eltrombopag (ELT) on Nitric Oxide Synthase (iNOS) and Cluster of Differentiation 206 (CD206) protein expression. (A) iNOS protein expression levels in Immune Thrombocytopenia (ITP) macrophages compared with CTR (control) macrophages, determined by Western Blot, starting from 20 μg of total lysates after treatment with Eltrombopag (6 μM). (B) CD206 protein expression levels in ITP macrophages compared with CTR macrophages, determined by Western Blot, starting from 20 μg of total lysates after treatment with ELT (6 μM). The most representative images are displayed. The proteins were detected using Image Studio Digits software (LI_COR Biotechnology ®, Lincoln, Nebraska USA)and the intensity of immunoblots compared to the untreated control, taken as 1 (arbitrary unit), were quantified after normalizing with respective loading controls for the housekeeping protein β-Tubulin. Histogram shows iNOS (A) and CD206 (B) expression levels as the mean ± S.D. of independent experiments on each individual sample. A t-test was used for statistical analysis. * indicates p ≤ 0.05 compared to Non-treated Control (CTR NT), ^ indicates p ≤ 0.05 compared to ITP NT.