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. 2020 Dec 27;22(1):185. doi: 10.3390/ijms22010185

Figure 7.

Figure 7

Binding of N-Ret-PE as a function of all-trans retinal concentration for selected TMD variants. ABCA4 TMD variants immobilized on an immunoaffinity matrix were treated with various concentrations of radiolabeled all-trans retinal. The amount of bound N-Ret-PE was determined after the removal of excess retinal. (A) Best fit binding curves for TMD1 variants (n ≥ 2) for the following apparent mean Kd ± SD; WT Kd = 1.2 ± 0.2 µM; p.T716M Kd = 6.3 ± 0.7 µM; p.C764Y Kd = 4.6 ± 0.4 µM; p.V849A Kd = 6.4 ± 0.9 µM; p.A854T Kd = 8.6 ± 0.9 µM. (B) Best fit binding curves for TMD2 variants for n ≥ 2; Kd = p.E1399K Kd = 2.8 ± 0.3 µM; p.S1696N Kd = 3.5 ± 0.3 µM; p.R1843W Kd = 31 ± 3.5 µM; p.N1868I Kd = 12.0 ± 0.8 µM; p.R1898C Kd = 16.0 ± 1.7 µM.