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. 2021 Jan 2;13(1):123. doi: 10.3390/cancers13010123

Figure 2.

Figure 2

Examples of HD FTIR (A) and RS images (B) constructed for integral intensities of bands specific for main cellular macromolecules and their clustering into false-color maps (Unsupervised Hierarchical Cluster Analysis (UHCA) and k-means cluster (KMC) for FTIR and Raman images, respectively). The colors in UHCA and KMC cluster maps correspond to nucleus (blue), cytoplasm (green), graininess in cytoplasm (red; present in all 20 cells of the four cell lines except HCV-29 and J82), and lipid droplets (orange; present in 18 out of 20 HCV-29 cells and 17 out of 20 T24p cells). Abbreviations: HD—high definition, NuAc—nucleic acids. Color bars (min–max) denote changes in intensities of bands chosen to construct chemical images.