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Averaged second derivatives of FTIR transmission spectra of the nuclei and cytoplasm for each cell line and graininess observed for T24a, T24p, RT4 and HT1376 cells compared to ATR−FTIR spectra of whole cells. Gray shading denotes standard deviation (±SD); N = 20 spectra per cell line. Averaged normal FTIR spectra are shown in Figure S3 in SM.
