Figure 3.

Cumulated heatmaps of receptor regulation (A), gene expression (B), protein abundance (C), and protein activity (D) of aryl hydrocarbon receptor (AHR) and its targets cytochrome P450 monooxygenase 1A1 (CYP1A1) and 1A2 (CYP1A2), respectively. Respective experiments were conducted as described in the Materials and Methods section. For protein abundance and activity, the following applied: For values below the respective lower limit of quantification (LLOQ), the value half of the LLOQ was used for calculations. Generally, mean values were calculated from the individually measured raw data. Fold changes with regard to the respective solvent control were determined and then log2-transformed (log2FC). A positive regulation is indicated by red shades, a negative regulation by blue shades. Statistical analysis was performed with Student’s t-test (n = 3, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Underlying fold changes and standard deviations can be found in the Supplementary Materials “Underlying Data”.