Table 2.
Relation of LAG-3, TIM-3 and VISTA expression in association with CD8-positive TILs according to each other in the whole cohort (n = 241) and according to human papillomavirus (HPV)-status (HPV-related oropharyngeal squamous cell carcinoma (OPSCC), n = 63, HPV-negative OPSCC, n = 177).
| LAG-3 Expression | TIM-3 Expression | VISTA Expression | CD8 Expression | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| All | yes | (%) | no | (%) | p | yes | (%) | no | (%) | p | yes | (%) | no | (%) | p | yes | (%) | no | (%) | p | |
| 73 | 31% | 166 | 69% | 122 | 51% | 119 | 49% | 168 | 70% | 73 | 30% | 89 | 37% | 149 | 63% | ||||||
| LAG-3 Expression | yes | 54 | 74% | 19 | 26% | <0.001 | 66 | 90% | 7 | 10% | <0.001 | 39 | 56% | 31 | 44% | <0.001 | |||||
| no | 68 | 41% | 98 | 59% | 101 | 61% | 65 | 39% | 50 | 30% | 116 | 70% | |||||||||
| TIM-3 Expression | yes | 113 | 93% | 9 | 7% | <0.001 | 65 | 54% | 55 | 46% | <0.001 | ||||||||||
| no | 55 | 46% | 64 | 54% | 24 | 20% | 94 | 80% | |||||||||||||
| VISTA Expression | yes | 71 | 43% | 94 | 57% | 0.007 | |||||||||||||||
| no | 18 | 25% | 55 | 75% | |||||||||||||||||
| CD8 Expression | yes | ||||||||||||||||||||
| no | |||||||||||||||||||||
| HPV-relation | yes | 28 | 44% | 35 | 56% | 0.006 | 44 | 70% | 19 | 30% | <0.001 | 47 | 75% | 16 | 25% | 0.313 | 42 | 67% | 21 | 33% | <0.001 |
| no | 45 | 26% | 130 | 74% | 78 | 44% | 99 | 56% | 120 | 68% | 57 | 32% | 46 | 26% | 128 | 74% | |||||
| HPV-related OPSCC | LAG-3 Expression | TIM-3 Expression | VISTA Expression | CD8 Expression | |||||||||||||||||
| n = 63 | yes | (%) | no | (%) | p | yes | (%) | no | (%) | p | yes | (%) | no | (%) | p | yes | (%) | no | (%) | p | |
| 28 | 44% | 35 | 56% | 44 | 70% | 19 | 30% | 47 | 75% | 16 | 25% | 42 | 67% | 21 | 33% | ||||||
| LAG-3 Expression | yes | 24 | 86% | 4 | 14% | 0.026 a | 25 | 89% | 3 | 11% | 0.021 a | 25 | 89% | 3 | 11% | 0.001 a | |||||
| no | 20 | 57% | 15 | 43% | 22 | 63% | 13 | 37% | 17 | 49% | 18 | 51% | |||||||||
| TIM-3 Expression | yes | 41 | 93% | 3 | 7% | <0.001 a | 37 | 84% | 7 | 16% | <0.001 a | ||||||||||
| no | 6 | 32% | 13 | 68% | 5 | 26% | 14 | 74% | |||||||||||||
| VISTA Expression | yes | 38 | 81% | 9 | 19% | <0.001 a | |||||||||||||||
| no | 4 | 26% | 12 | 74% | |||||||||||||||||
| CD8 Expression | yes | ||||||||||||||||||||
| no | |||||||||||||||||||||
| HPV-negative OPSCC | LAG-3 Expression | TIM-3 Expression | VISTA Expression | CD8 Expression | |||||||||||||||||
| n = 177 | yes | (%) | no | (%) | p | yes | (%) | no | (%) | p | yes | (%) | no | (%) | p | yes | (%) | no | (%) | p | |
| 45 | 26% | 130 | 74% | 78 | 44% | 99 | 56% | 120 | 68% | 57 | 32% | 46 | 26% | 128 | 74% | ||||||
| LAG-3 Expression | yes | 30 | 67% | 15 | 33% | 0.001 | 41 | 91% | 4 | 9% | <0.001 a | 14 | 33% | 28 | 67% | 0.267 | |||||
| no | 48 | 40% | 82 | 60% | 78 | 60% | 52 | 40% | 32 | 25% | 98 | 75% | |||||||||
| TIM-3 Expression | yes | 72 | 92% | 6 | 8% | <0.001 | 28 | 37% | 48 | 63% | 0.006 | ||||||||||
| no | 48 | 48% | 51 | 52% | 18 | 18% | 80 | 82% | |||||||||||||
| VISTA Expression | yes | 32 | 27% | 85 | 73% | 0.695 | |||||||||||||||
| no | 14 | 25% | 43 | 75% | |||||||||||||||||
| CD8 Expression | yes | ||||||||||||||||||||
| no | |||||||||||||||||||||
p-values calculated by x² test (Pearson, asymptotic, two-sided) or a exact test (Fisher, two-sided), significant p-values (p≤ 0.05) in bold.