Table 1.
Median normalized expression levels of ADRN and MES panel by multiplex RT-qPCR in control BM (n = 54) and control PB (n = 50).
| Marker | BM | PB | ||||
|---|---|---|---|---|---|---|
| Positive BM Samples | Expression (Median + IQR) | Threshold | Positive PB Samples | Expression (Median + IQR) | Threshold | |
| PHOX2B | 0/54 | 0/50 | ||||
| TH | 17/54 | 16.0 (0.9) | 13.0 | 21/50 | 14.3 (0.9) | 11.3 |
| CHRNA3 | 42/54 | 16.0 (1.7) | 13.0 | 21/50 | 16.4 (1.7) | 13.4 |
| GAP43 | 42/54 | 17.0 (2.3) | 14.0 | |||
| DBH | 6/50 | 16.7 (0.7) | 13.7 | |||
| FMO3 | 54/54 | 8.6 (2.7) | 16/50 | 15.2 (1.4) | ||
| PRRX1 | 54/54 | 10.3 (2.8) | 9.0 * | 30/50 | 15.3 (1.7) | 12.3 ** |
| POSTN | 54/54 | 12.1 (3.0) | 9.0 * | 27/50 | 15.4 (2.0) | 12.5 ** |
All samples represent the median (±interquartile range (IQR)) of normalized Ct values (ΔCt = Ctmarker – CtGUSB). * Threshold for PRRX1 in BM is defined as ΔCt < 9.0 and CtPRRX1 − CtFMO3 < −1. Threshold for POSTN in BM is defined as ΔCt < 9.0 and CtPOSTN − CtFMO3 < 1. ** Threshold for PRRX1 and POSTN in PB is defined as ΔCt < 12.3 and 12.5 respectively, and no expression of FMO3. BM = bone marrow, PB = peripheral blood.