Statins induced a concentration−dependent inhibition of mitochondrial respiration in permeabilized human platelets. (A) Representative traces of atorvastatin 40 µM (blue) and DMSO (grey). Concentration-dependent effects were assessed for 3 concentrations (40 µM, 80 µM and 160 µM, respectively) of atorvastatin (open circle), cerivastatin (open triangle) and simvastatin (open square), respectively. OXPHOS coupling efficiency (B), LEAK (C), maximal ET capacity (D), maximal OXPHOS (E), NADH-linked OXPHOS (F), and succinate-linked OXPHOS (G) capacities were evaluated. Data is expressed as mean±SEM of the percent of control (platelets exposed to the corresponding volume of DMSO for each of the 3 concentrations of statin). Two-way ANOVA with Bonferroni post hoc test was performed on antimycin-corrected data. DMSO, dimethyl sulfoxide; ET, electron transport; LEAK, non-phosphorylating resting stat; OXPHOS, oxidative phosphorylation; ROX, residual oxygen consumption. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 vs. DMSO.