Figure 1.

Dihydrotestosterone (DHT) treatment reduces brown adipogenesis. (A) Representative images showing presence of the androgen receptor protein in brown preadipocytes and adipocytes. Blue—nuclear stain DAPI, green—androgen receptor. (B) Representative differential interference contrast (DIC) microscopy images showing a reduction of lipid accumulation and brown adipocyte differentiation with increasing dose of DHT. (C) Representative images showing a reduction in lipid droplet formation with DHT, measured by Oil Red O in control and the 10 μM DHT. (D) Relative mRNA expression of adipogenic markers in brown adipocytes differentiated for seven days in the presence of different doses of DHT (t-test, * p < 0.05. Data shown is mean (±SEM n = 4).