Table 1.

Effects of the different PEF protocols on the human melanotic A375 melanoma cells.

	Permeability of the Membrane during PEF Treatment	Permeability of the Membrane after PEF Treatment	Long-Term Reduced Viability	ROS Burst	Lipid Oxidation	Change in the Plasma Membrane Integrity	Changes in the Membranes of the Internal Organelles
IRE protocols (2–4 kV/cm, 0.1 ms, 8 pulses).	YES	HIGH	YES	YES, voltage-dependent	HIGH, voltage-independent	YES	YES
(200–400 V/cm, 0.1 ms, 8 pulses).	NO (same as control)	NO	NO	NO	LOW	NO	NO
(200–400 V/cm, 10 ms, 8 pulses).	NO (same as control)	LOW	NO	YES, voltage-independent	HIGH, voltage-dependent	NO	YES, 400 V/cm PEF exclusively