Skip to main content
. 2021 Jan 4;14(1):208. doi: 10.3390/ma14010208

Figure 3.

Figure 3

Effect of L-tryptophan on the stem cell phenotype of mBMSCs and trabecular bone in vivo. (A) FCM analysis of the percentage of mBMSCs positive for SSEA-4. mBMSCs from L-Trp (50 mg/kg)-treated mice showed a significantly higher number of SSEA-4 positive cells than the control group. * p < 0.05, one-way ANOVA/Turkey. (B) Gene expression levels of Nanog and Oct-4 in mBMSCs from 50 mg/kg L-Trp-treated mice was significantly higher than mBMSCs from the PBS-injected control group. Data represent the mean ± SD (n = 3). * p < 0.05, *** p < 0.001, unpaired t-test. (C) Treatment with L-Trp increased the colony-forming ability of mBMSCs. Data represent the mean ± SD (n = 3). * p < 0.05, unpaired t-test. (D,E) Differentiation ability. L-Trp induced stronger deposition of the calcified matrix as detected by Alizarin Red S staining (D) as well as an increase in the mRNA levels of Opn and Ocn on mBMSCs (E). No substantial change in Oil Red O staining (F) or gene expression levels of Ppar-γ or Lpl (G) could be observed upon L-Trp treatment. * p < 0.05, *** p < 0.001, unpaired test. Images and graphs are representative of at least three independent experiments.