Figure 9.

qRT-PCR (Quantitative Real-time Polymerase Chain Reaction) analysis of 40 randomly selected genes (A) pericarp; (B) aril. Lc-EF-1α and Lc-Atcin were used as reference genes for normalization of gene-expression data. Red and blue bars represent the data yielded by qRT-PCR in ‘Nuomici’ and ‘Huaizhi’, respectively. Red and blue lines represent the data yielded by RNA sequencing in ‘Nuomici’ and ‘Huaizhi’, respectively. Error bars indicate standard errors of the means (n = 3). The full name of each gene abbreviation is MLO: Mildew resistance locus O, CML: Calmodulin-like protein, PIP: Aquaporin, ACP: Acyl carrier protein; ARF: Auxin response factor, CIPK: CBL-interacting serine/threonine-protein kinase, CGNC: Cyclic nucleotide-gated ion channel, PAEI: Pectinesterase inhibitors, PPR: Pentatricopeptide repeat-containing protein, BAK: brassinosteroid-insensitive associated receptor kinase, STK: Serine/threonine-protein kinase, TPS: Trehalose-6-phosphate synthase, TPP: Trehalose-phosphate phosphatase, XPT: Xylulose 5-phosphate translocate, EG: endo-1,4-β-glucanase.