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. 2021 Jan 5;22(1):460. doi: 10.3390/ijms22010460

Figure 3.

Figure 3

Immunofluorescence staining showing signal transducer and activator of transcription 3 (STAT3) and GA-binding protein alpha (GABPα) expression and localization in mouse zygotes and 2-cell stage embryos. Localization and expression of two transcription factors, STAT3 (A) and GABPα (B), in zygote stage embryos 20 h post-hCG and 2-cell stage embryos 40 h post-hCG. DAPI: 4′,6-diamidino-2-phenylindole, a blue-fluorescent dye that binds to AT-rich regions of double-stranded DNA and is used for nuclear localization staining. The abundance of green fluorescence representing the expression and localization of transcription factors STAT3 (zygote, n = 5 and 2-cell embryo, n = 6) and GABPα (zygote, n = 5 and 2-cell embryo, n = 6) in early-stage mouse embryos. Scale bar = 20 µm. Quantitative data of the fluorescence intensities of STAT3 (C) and GABPα (D) located in the zygotic pronuclei or 2-cell stage embryonic nuclei. The asterisk (*) indicates a significant difference between two pronuclei (*, p < 0.05 and **, p < 0.01) in each embryo as analyzed by Student’s t-test.