Skip to main content
. 2021 Jan 5;22(1):460. doi: 10.3390/ijms22010460

Figure 4.

Figure 4

STAT3 accumulates in the pronuclei and nuclei after microinjection of the Δ4-Gzmg promoter construct plasmid in mouse zygotes and 2-cell stage embryos. (A) Plot profile yellow lines represent the abundance and localization of transcription factors STAT3 (left panel) and GABPα (right panel), with or without Δ4-Gzmg promoter construct plasmid injection into one side of the zygotic pronuclei and 2-cell embryonic nuclei. Yellow arrowheads indicate nuclei after Δ4-Gzmg promoter construct plasmid injection. White arrowheads indicate nuclei without Δ4-Gzmg promoter construct plasmid injection. Scale bar = 10 µm. (B) Quantitative data were calculated by absolute difference values of fluorescence intensities of STAT3 (zygote, n = 5 and 2-cell embryo, n = 6) and GABPα (zygote, n = 6 and 2-cell embryo, n = 5) between the Δ4-Gzmg promoter plasmid-injected nucleus and the noninjected nucleus from the same zygote or 2-cell stage embryo. The asterisks (** and ***) indicate a significant difference as determined by Student’s t-test. (**, p < 0.01 and ***, p < 0.001).