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. 2021 Jan 5;22(1):460. doi: 10.3390/ijms22010460

Figure 6.

Figure 6

A novel N-terminal domain (NTD)-deleted STAT3 in the pronucleus of mouse zygote stage embryos. (A) A graphic hypothesis showing that the N-terminal domain (NTD) deletion of STAT3 may be detected by two different recognition domains of STAT3 antibodies. One is labeled with an anti-F-2 antibody that specifically recognizes the N-terminal domain of STAT3 and is used for full-length STAT3α detection. The other is an anti-C-20 antibody that specifically recognizes the C-terminal domain of STAT3 and is used for the detection of both NTD-deleted STAT3 and STAT3α. (B) Immunolocalization of NTD-deleted STAT3 and full-length STAT3α in mouse zygote stage embryos by anti-F-2 (red fluorescent color) and anti-C-20 (green fluorescent color) antibodies. DNA was stained with DAPI (blue fluorescent color) to define pronuclei localization. Scale bar = 10 µm.