Table 2.
Development rates of mouse embryos at E3.5 and transcription activities of the Gzmg promoter by culturing with STAT3 inhibitors.
| Different Inhibitor Treatment | Total Embryos | No. (%) of Embryos Developed to | Transcription Activity (Relative Fluorescence Unit) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Zygote | 2-Cell Embryo | 4-Cell Embryo | Morula Embryo | Blastocyst Embryo | Fragment Embryo | Mean | S.D. | p-Value | p-Value | ||
| (w/DMSO) | (w/KSOM) | ||||||||||
| S3I-201 (100 µM) | 44 | 15 (34.1) | 29 (65.9) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 3.193 | 0.788 | 0.0552 | 0.0372 |
| WP1066 (5 µM) | 57 | 0 (0.0) | 18 (31.6) | 0 (0.0) | 0 (0.0) | 39 (68.4) | 0 (0.0) | 3.746 | 1.14 | 0.1283 | 0.0967 |
| DMSO 1 (1/1000) | 56 | 0 (0.0) | 1 (1.8) | 0 (0.0) | 0 (0.0) | 50 (89.3) | 5 (8.9) | 5.628 | 1.722 | - | 0.9921 |
| KSOM 2 (Control) | 59 | 0 (0.0) | 2 (3.4) | 0 (0.0) | 0 (0.0) | 56 (94.9) | 1 (1.7) | 5.639 | 2.158 | 0.9921 | - |
1 DMSO: dimethyl sulfoxide; 2 KSOM: KSOM-AA culture medium.