Figure 3.

Relative levels of transcripts of selected TEs in seedlings of A. thaliana plants exposed repeatedly to 250 µM zebularine during germinations and in leaves of plants grown from these seedlings. RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel) from 100 mg of 7-day-old seedlings and leaves of 8-week-old plants. Relative levels of transcripts in Z/Z/Z 7-day-old seedlings (7ds) and leaves were compared to levels detected in control organs (7ds O/O/O, leaf O/O/O/, for experimental design and nomenclature of samples, see Figure 1). S, plants with short telomeres and L, plants with long telomeres (see Figure 2a). Signals of TE transcripts were related to that of the ubiquitin10 reference gene, levels of TE transcripts in 7ds O/O/O samples were arbitrarily chosen as 1. Data were evaluated by two sample F-test and t-test. * p < 0.05, ** p < 0.01, and *** p < 0.001. Note the logarithmic scale at the y axis.