Table A1.
MRM transitions for LC-MS/MS analysis of indolic species. Each subclass is preceded by the correspondent IS (in italics) used for quantification.
| Name | MW | Q1 | Q3 | DP | CE | Rt |
|---|---|---|---|---|---|---|
| 5F TRY(IS1) | 178 | 179.2 | 162.2 | 20 | 15.2 | 3.89 |
| TRY | 160 | 161.2 | 144.2 | 20 | 14.8 | 2.89 |
| 5OH TRY (or Ser) | 176 | 177.2 | 160.2 | 20 | 14.7 | 1.30 |
| 5OCH3 TRY | 190 | 191.2 | 174.2 | 20 | 14.3 | 3.13 |
| NAc TRY | 202 | 203.2 | 144.2 | 20 | 19.1 | 6.87 |
| NAc 5OHTRY (or N-Ac SER) | 218 | 219.2 | 160.2 | 20 | 18.5 | 3.71 |
| TRP D5 (IS2) | 209 | 210.2 | 192.2 | 20 | 14.8 | 3.03 |
| 3OH AA | 153 | 154.1 | 135.9 | 21 | 17.0 | 2.91 |
| 3OH KYN | 224 | 225.1 | 208.1 | 16 | 13.0 | 1.30 |
| AA | 137 | 138.1 | 119.8 | 21 | 15.0 | 5.63 |
| KYNA | 189 | 190.1 | 89.1 | 36 | 51.0 | 4.56 |
| KYN | 208 | 209.2 | 192.2 | 21 | 15.0 | 1.72 |
| TRP | 204 | 205.2 | 188.2 | 20 | 13.4 | 3.12 |
| TEE | 232 | 233.2 | 216.2 | 20 | 13.1 | 6.06 |
| 5OH TRP | 220 | 221.1 | 204.2 | 16 | 15.0 | 1.60 |
| MEL OCD3 (IS3) | 235 | 236.2 | 177.2 | 20 | 19.1 | 6.80 |
| MEL | 232 | 233.2 | 174.2 | 20 | 18.8 | 6.84 |
MW: molecular weight, Q1: molecular ion, Q3: daughter ion, DP: declustering potential, CE: collision energy, Rt: retention time.