Fig. 4.

Exposure to diesel exhaust particles results in increased expression of IL-10. Representative images of Interleukin - 10 (IL-10) expression in the lungs of C57Bl/6 mice on a low-fat (LF; a-c or high-fat (HF; g-i) diet exposed to saline (control) or on a LF (d-f) or HF diet (j-l) exposed to diesel exhaust particles (DEP – 35 μg PM) twice a week for a total of 30 days. Red fluorescence indicates IL-10 expression, blue fluorescence is nuclear staining (Hoechst). Right panels (c, f, i, l) are merged figures of left (blue; a, d, g, j) and center (red; b, e, h, k) panels. m Graph of histology analysis of lung IL-10 fluorescence and n mean normalized gene expression of IL-10 mRNA transcript expression within the lung, as determined by RT-qPCR. 40x magnification, scale bar = 100 μm. *p < 0.05 compared to LF Control, †p < 0.05 compared to HF Control, ‡p < 0.05 compared to LF DEP by two way ANOVA