Fig. 5.

Exposure to diesel exhaust particles results in increased mucus production. a Mean normalized gene expression of Muc5b, b Muc5ac and c Muc4 mRNA transcript expression within the lungs of C57Bl/6 wildtype mice, as determined by RT-qPCR, on either control (LF) or high-fat (HF) diet, exposed to either saline (control) or diesel exhaust particles (DEP − 35 μg PM) twice a week for a total of 30 days. d Representative images of AB/PAS staining of lung sections and e quantification of histological mucus score. Images displayed are using 20X magnification, scale bar = 240 μm. Data are depicted as mean ± SEM with *p < 0.05 compared to LF Control, ‡p < 0.05 compared to LF DEP by two way ANOVA