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. 2021 Jan 9;22:5. doi: 10.1186/s12865-020-00389-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Sera from ZIKV seropositive donors effectively neutralizes ZIKV infection. Vero cells were infected with (a) ZIKV (b) WNV or (c) DENV GFP RVPs in the presence of media or serial dilutions of a WNV+ serum sample (symptomatic) or ZIKV+ serum samples obtained from BEI resources. The number of GFP positive cells was determined 72 h post infection. All wells were assayed in duplicates and percent infection determined after normalizing data to 100% infection in the absence of any sera. K562 cells were infected with (d) ZIKV (e) WNV or (F) DENV GFP RVPs in the presence of serial dilutions of a WNV+ serum sample (symptomatic) or ZIKV+ sera samples. The number of GFP positive cells was analyzed 48 h post infection and normalized to peak ADE as 100% infection. g Serum dilution that causes 50% neutralization of ZIKV, WNV and DENV RVPs was determined for each sample. h Serum dilution that causes peak ADE for WNV, ZIKV and DENV RVPs was determined