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. 2021 Jan 9;22:5. doi: 10.1186/s12865-020-00389-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Prevalence of cross reactive E antibodies correlates with ZIKV neutralization and enhancement. a Stable cell line expressing Zika CprME-NS2B3 was radiolabeled with [³⁵S]Met/Cys and cell lysates immunoprecipitated with Protein-A agarose beads coated with different WNV+ sera samples. Samples were resolved on and SDS-PAGE gel followed by PhosphorImager analysis. b Correlation analysis of Envelope band intensity with serum dilution causing 50% ZIKV neutralization. c Correlation analysis of Envelope band intensity with dilution of peak ZIKV ADE. Curves were fitted using GraphPad prism and simple linear regression. P values were obtained from two-tailed t test