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. 2021 Jan 9;21:21. doi: 10.1186/s12866-020-02082-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Analysis of motility and biofilm phenotypes from A. baumannii strains isolated from sputum or bronchial wash samples. a Motility agar plates 24 h post-inoculation with A. baumannii clinical isolate strains. b Crystal violet stained A. baumannii biofilms in polystyrene tubes. c Quantitative analysis of bacterial motility as determined by measurement of diameter of bacterial cells present on the plate. Bars indicate mean values (+/− standard error mean error bars) with individual biological replicates indicated by discrete points (n=4). d Quantitative analysis of ratio of biofilm to biomass. Biofilm was determined by solubilization of crystal violet and spectrophotometric measurement at OD₅₆₀. Biomass was determined by spectrophotometric measurement at OD₆₀₀. Bars indicate mean values (+/− standard error mean error bars) with individual data points indicated by discrete points (n=3 biological replicates with 3–4 technical replicates per experiment)