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. 2020 Dec 9;184(1):120–132.e14. doi: 10.1016/j.cell.2020.12.006

Figure 1.

Figure 1

Genome-wide CRISPR Screens Identify Host Factors Required for SARS-CoV-2 Infection

(A) Genome-wide CRISPR screening workflow. Cas9-expressing Huh-7.5 cells are transduced with the Brunello genome-wide CRISPR library, selected with puromycin, and infected with SARS-CoV-2 or one of three seasonal CoVs (HCoV-OC43, HCoV-NL63, or HCoV-229E). Surviving cells and mock controls are then harvested, and sgRNA abundance is determined using next-generation sequencing.

(B) Bubble plot of data from SARS-CoV-2 screens at 37°C. Red lines denote z = ±2.

(C) Bubble plot of data from SARS-CoV-2 screens at 33°C. Red lines denote z = ±2.

(D) Scatterplot comparing Z scores from (B) and (C) for SARS-CoV-2 screens at 37°C and 33°C, respectively.

(E) Subset of significantly enriched genes from SARS-CoV-2 screens at 37°C and 33°C.