Skip to main content
. Author manuscript; available in PMC: 2022 Jan 15.
Published in final edited form as: Neuroscience. 2020 Nov 14;453:168–186. doi: 10.1016/j.neuroscience.2020.10.025

Fig 6: A detailed assessment of astrogliosis in CA1 of the hippocampus in epileptic and D-serine treated animals.

Fig 6:

A, Schematic of MEA-hippocampal brain slice showing subdivisions of the CA1 subfield and the extent of astrogliosis in the various subdivisions.

B, Representative examples of density grams for astrocytes, generated by plotting the precise location of cells counted on a grid in various subdivisions of CA1 (c, blue; b, red and a, black) under the indicated conditions. Note the upregulation of astrocytic density in CA1c in epileptic animals.

C, Histogram of averaged astrocytic density across the entire CA1 subfield under the indicated conditions. Data within bar plots indicate number of animals used (n) and error bars indicate SEM. The key (inset) pertains to non-status controls, epileptic and D-serine-treated animals in this and all subsequent histograms. **** p < 0.0001, ** p < 0.01, ns, not significant.

D, Histograms of averaged astrocytic densities across various subdivisions of CA1 under the conditions indicated in the bar plots (non-status controls, epileptic and D-serine-treated; key in C). Data within bar plots indicates number of animals (n) used (numerator) and the total number of sections assayed for each condition (denominator). Error bars indicate SEM. Unless otherwise indicated, in this and subsequent figures, p values are determined with either a parametric or non-parametric (np) 1- or 2-way ANOVA and where appropriate, t-test with Welch’s correction or an unpaired (np) Mann-Whitney test. *** p < 0.001, **** p < 0.0001, ns, not significant.

E, Histogram of averaged astrocytic density across individual subdivisions of the CA1 subfield under the indicated conditions. Data within bar plots indicate number of animals used (n) and error bars indicate SEM. **** p < 0.0001, ns, not significant.

F, Histogram of averaged astrocytic density in epileptic and D-serine treated animals in the indicated subdivisions of the CA1 subfield. Data within bar plots indicate number of animals used (n) and error bars indicate SEM. *** p < 0.001, ns, not significant.

G, Histograms of averaged astrocytic densities in the hilus under the indicated conditions (non-status controls, epileptic and D-serine-treated; key in C). Data within bar plots indicates number of animals (n) used (numerator) and the total number of sections assayed for each condition (denominator). Error bars indicate SEM. **** p < 0.0001, ns, not significant.