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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biotechnol Lett. 2020 Sep 9;43(1):13–24. doi: 10.1007/s10529-020-02997-9

Fig. 2. Example of the double RISH procedure for transcripts of the mechanotransduction components YAP, TAZ and LMNA on sections through the coccygeal bovine IVD.

Fig. 2

(a-d) shows cells of the AF. (e-h) shows cells of the NP. Alexa-488 conveyed fluorescence reflects a RNA probe targeting exon2 of LMNA in (a,c,e and g) or the TAZ transcript in (b,d,f and h). Alexa-594 conveyed fluorescence indicates the presence of a RNA probe targeting exon 13 of LMNA in (a,d,e and h) or the YAP transcript in (b,c,f and g). The white arrowhead in (h) points to a potential progenitor cell as indicated by the absence LMNA or TAZ transcripts. DAPI stains the nucleus