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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biotechnol Lett. 2020 Sep 9;43(1):13–24. doi: 10.1007/s10529-020-02997-9

Fig. 3. Example of a combined RISH and IHC procedure for cell cycle markers Ki67 and CDK4 on primary bovine NP cells in culture.

Fig. 3

Alexa-488 conveyed fluorescence reflects the bovine Ki67 transcript, Alexa-568 conveyed fluorescence reflects the presence of Ki67 protein, while Alexa-647 conveyed fluorescence indicates presence of CDK4 protein. DAPI stains the nucleus. The white arrowhead in (e) points to a NP cell in metaphase. The green and red arrowheads in (f-j) point to Ki67 transcript and Ki67 protein, respectively