. Author manuscript; available in PMC: 2022 Jan 1.

Published in final edited form as: Biotechnol Lett. 2020 Sep 9;43(1):13–24. doi: 10.1007/s10529-020-02997-9

Table 2.

Primers used for the generation of bovine RISH probes used in this study

Name	Primer	PCR product	Sequence ID
LMNA exon 2	5’-CTATTAGAGCCTTTGCCCAGGA-3’	585 bp	XM_005203621.1
LMNA exon 2	^*5’-CCTTGAACTCCTCTCGCACT-3’	585 bp	XM_005203621.1
LMNA exon 13	5’-GCATCATGTAACCTGGGACCT-3’	858 bp	XM_005203621.1
LMNA exon 13	^*5’-GCAAGGGGCTCCTTAGTGTT-3’	858 bp	XM_005203621.1
YAP	5’-GCCGCCACCAAGCTAGATAA-3’	1160 bp	XM_024975708.1
YAP	^*5’-ACACTACCCCAACCGGATTT-3’	1160 bp	XM_024975708.1
TAZ	5’-TCCAGCTGCCTTTTGGACTT-3’	518 bp	XM_002699714.5
TAZ	^*5’-TTGGACTGGGCTCCCCTTAT-3’	518 bp	XM_002699714.5
Ki67	5’-CGAGCCTCAGAGCTGAAGTG-3’	915 bp	XM_015469655.1
Ki67	^*5’-GACTGGCTCCGGTTGAGAAG-3’	915 bp	XM_015469655.1

The following sequence was added to the 5’end of this primer to allow for T7-RNA polymerase driven in vitro transcription to generate an antisense probe: 5’CCAAGCTTGTAATACGACTCACTATAGGGC-3’