Figure 2.

The effect of XIST knockdown through transfection of LSMC spheroids with siRNA against XIST for 96 hours on XIST expression (A; N=7), cell proliferation (B; N=3), expression of miR-29c and miR-200c (C; N=7), and protein levels of COL1A1, COL3A1 and FN1 determined by western blot (D; N=6) along with the relative band density analysis (E). The results shown in (D) are representative of six sets of independent experiments and presented as mean ± SEM using cells isolated from different patients in each set. P values (*: p<0.05) are indicated by the corresponding lines.