Skip to main content
. Author manuscript; available in PMC: 2021 Feb 28.
Published in final edited form as: Mucosal Immunol. 2020 Aug 29;14(1):253–266. doi: 10.1038/s41385-020-00342-x

Figure 2. The vast majority of Mtb-infected pulmonary parenchymal and alveolar mononuclear myeloid cells express HO-1.

Figure 2.

(A) Representative dot plots showing mCherry M. tuberculosis staining in alveolar macrophages and parenchymal / alveolar (CD45 IV−) and intravascular (CD45 IV+) neutrophils (Neut) and mononuclear myeloid cells (MMC) from lungs of M. tuberculosis-infected mice at 4 weeks post infection (wpi), gated as detailed in Fig. S1 (flow cytometry data concatenated from 4 samples); (B) Percentage represented by each cell population among parenchymal / alveolar (IV−) HO-1+ cells isolated from naïve, or M. tuberculosis-infected mice at 2 and 4 wpi (n = 4 mice/group); (C) Representative dot plot showing phenotypic characterization by flow cytometry of parenchymal / alveolar (IV−) HO-1+ MMC (gate R1) isolated from lungs of mice at 4 wpi with M. tuberculosis (flow cytometry data concatenated from 4 samples); (D) HO-1 and beta actin detection by western blotting (bottom) in parenchymal / alveolar (IV−) cells sorted from lungs of mice at 4 wpi according to phenotypic characteristics detailed in Fig. S1 and Fig. 2C and quantification of HO-1 in each cell population normalized to beta actin (endogenous control) expression on the same samples (top) (n = 4 mice/group – pooled samples); (E) Percentage represented by Mtb-infected (black) or uninfected (gray) cells among HO-1+ AM and parenchymal / alveolar (IV−) Neut, CD11b+Ly6C− and CD11b+Ly6C+ MMC (n = 4 mice/group); (F) Frequency of HO-1+ cells among Mtb-infected AM and parenchymal / alveolar (IV−) Neut, CD11b+Ly6C− and CD11b+Ly6C+ MMC (n = 4 mice/group); (G) Representative dot plots showing HO-1 mCherry-Mtb and HO-1 staining in total AM and and parenchymal / alveolar (IV−) Neut, CD11b+Ly6C− and CD11b+Ly6C+ MMC populations (flow cytometry data concatenated from 4 samples). Data expressed as mean ± standard error of mean (B, E and F), dot plots from concatenated data (A, C and G) or pooled samples (D). Data shown are representative of 2 (A, E, F and G) or 3 independent experiments (B, C and D).