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. Author manuscript; available in PMC: 2022 Jan 15.
Published in final edited form as: Acta Biomater. 2020 Oct 29;120:224–239. doi: 10.1016/j.actbio.2020.10.011

Fig 6. Electron Microscopy images of the secreted ECM by DPSC, DPSC-STIM1OE and DPSC-STIM1KO cells.

Fig 6.

Fig 6.

Fig 6.

Fig 6.

(A) Representative FESEM images showing the presence of vesicles in the ECM secreted by the 3 cell types (A1–A3) when cultured under growth media conditions for 14 days.

(B) Representative FESEM images showing mineral deposition by the 3 cell types (B1–B3) cultured under growth conditions for 14 days.

(C) Representative FESEM images showing the presence of vesicles in the ECM deposited by 3 cell types (C1–C3) when cultured for 14 days under differentiation conditions.

(D) Representative FESEM images showing the deposition of mineral in the ECM of the 3 cell types (D1–D3) when cultured for 14 days under differentiation conditions.

(E) Number of vesicles secreted by the 3 cell types under different culture conditions. Statistical significance as indicated. **p<0.01; *p<0.05.

(F) Spatial distribution of Pi (Cyan map) and Ca 2+ (Blue map) as obtained by EDX analysis of the mineral deposited by DPSC-STIM1OE cells (F1–F3). Element spectrum and composition (F4 & F5).