Fig. 6.

Evaluation to dark-induced senescence and blast resistance of OsNBL1 OE plants. a-b The phenotype of WT, nbl1, OE-4 and OE-6 plants at seedling stage (a) and heading stage (b). c Detached leaves of WT, OE-4 and OE-6 plants grown for six-weeks were immersed in buffer solution (3 mM MES, pH 5.8) at 28 °C for 5 days with complete darkness. d Total chlorophyll of the flag leaves of WT, OE-4 and OE-6 plants were measured at heading stage. Data are means ± SD (n=3). **P < 0.01 (Student’s t-test). e-g Punch (e) and spray (g) inoculation of WT, nbl1, OE-4 and OE-6 plants with a virulent M. oryzae isolate H535. Photograph and measurement of lesion length (f) was at 96 hpi