Figure 7.

MMEJ-targeting of double-strand break sites for functional genomics and gene therapies. MENdel provides genome engineers with the largest prediction coverage of single majority frameshifts for loss-of-function experiment design (boxed). We sampled 54 vertebrate genes for knockout-generating PreMAs using MENTHU and MENdel, and estimated that the majority (∼90%) of vertebrate genes should possess at least one early out-of-frame single majority outcome. MENTHU (right) is the only double-strand break repair prediction algorithm that allows DNA targeting with nucleases different to SpCas9 and offers scientists with customizable prediction thresholds to best accommodate user needs.