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. 2020 Dec 8;49(1):38–52. doi: 10.1093/nar/gkaa1164

Table 1.

Identification of characteristic products of RNase A digested tRNAphe in HeLa and HeLa/T cells

Digestion products Precursor ions (m/z) a type ions c type ions w type ions y type ions
[Gm]AA[OHyW]AΨ [M-3H]3−751.1259 a1 N.D c1+H2O 376.0658 w1 402.9962 y1 323.0271
(HeLa Fragment) [M-2H]2−1127.19146 a2 N.D c2 687.1061 w2 732.0464 y2 652.0797
Position 34–39 a3 N.D c3 1016.1574 w3 N.D y3-OHyW 846.0765
a4-OHyW 1130.1880 c4-OHyW 1210.1584 w4 N.D y4 N.D
a5 N.D c5 N.D w5 N.D y5 N.D
[Gm]AA[imG-14]AΨ [M-3H]3- 683.4321 a1 N.D c1+H2O 376.0650 w1 402.9951 y1 323.0288
(HeLa/T Fragment-1) [M-2H]2− 1025.6513 a2 N.D c2 687.1054 w2 732.0454 y2 652.0791
Position 34–39 a3 N.D c3 1016.1587 w3 N.D y3 1035.1394
a4-[imG-14] 1130.1920 c4 1400.2192 w4 N.D y4 N.D
a5 N.D c52− 864.1325 w5 N.D y5 N.D
[Gm]AA[imG/imG2]AΨ a1 N.D c1+H2O 376.0665 w1 402.9932 y1 323.0273
(HeLa/T Fragment-2) [M-3H]3- 688.1031 a2 N.D c2 687.1060 w2 732.0461 y2 652.0808
Position 34–39 [M-2H]2− 1032.6588 a3 N.D c3 1016.1593 w3 N.D y3- imG/imG2 846.0762
a4-imG 1130.1880 c4-imG/imG2 1210.1598 w4 N.D y4 N.D
a5 N.D c5 N.D w5 N.D y5 N.D

N.D: not detected