Figure 2.

NanoSIMS imaging of ³⁴S-labeled and Br-labeled ASOs in HeLa cells. HeLa cells were incubated in medium containing (A) ³⁴S-ASO or (B) Br-ASO for 24 h and then processed for NanoSIMS analyses. ¹²C¹⁴N images were useful for defining cell morphology; ³⁴S/³²S and ⁷⁹Br NanoSIMS images show distributions of ³⁴S-ASO and Br-ASO, respectively. Two ⁷⁹Br images, with different scales, are shown to depict the relative distributions of the Br-ASO in the cytoplasm and nucleus. The ³⁴S/³²S ratio scale is multiplied by 10 000. N, nucleus; C, cytoplasm.