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. 2020 Dec 8;49(1):114–126. doi: 10.1093/nar/gkaa1130

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Histone benzoylation is an inducible epigenetic mark in cells and mice. (A) Western blot analysis of core histone Kbz levels in response to the indicated concentrations of NaBz treatment in HEK 293T and HCT116 cells. Unmodified histone H4 was used as a loading control. (B) Immunofluorescence analysis of Kbz levels in HEK 293T and HCT116 cells. DNA was stained with DAPI. Control, cells without NaBz treatment. (C) Western blot analysis of core histone Kbz and Kac levels in mouse intestinal epithelial cells after the indicated concentrations of NaBz water feeding. Unmodified histone H4 was used as a loading control. Assays were performed in duplicates by using two mice for each concentration.