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. 2020 Dec 11;49(1):383–399. doi: 10.1093/nar/gkaa1180

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Fil1 is the major translational responder to stress. (A) Comparison of translation efficiency of the fil1 gene between stressed and control cells (log₂ ratios stress/control). The dotted lines indicate 1.5-fold changes. Data are presented for wild type, eIF2α-S52A and sty1Δ cells. (B) As in C, but for fil1 mRNA changes. The dotted lines indicate 2-fold changes. (C) Western blots to measure Fil1-TAP protein levels after cadmium treatment for the indicated times. Data are presented for wild type, eIF2α-S52A and sty1Δ cells. Tubulin was used as a loading control. (D) As in C, but after H₂O₂ treatment.