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. 2020 Dec 11;49(1):383–399. doi: 10.1093/nar/gkaa1180

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Oxidative stress affects the translation efficiency of tryptophan codon-enriched genes, and decreased tRNA-Trp charging may affect eIF2α phosphorylation. (A) Western blots to investigate the effect of tryptophan on eIF2α phosphorylation levels after H₂O₂ treatment. Cells were treated with H₂O₂ for 15 min and supplemental tryptophan was added as indicated. Tubulin was used as a loading control. (B) Quantification of eIF2α phosphorylation normalized to total eIF2α from the experiment shown in A (n = 4 independent replicates). (C) Boxplots showing changes in translation efficiency upon oxidative stress (log₂ TE ratios stress/control, 15 min treatment) according to tryptophan codon content. Genes were binned into 11 categories based on the fraction of tryptophan codons in their coding sequences (the first group contains 269 genes without tryptophan, codons and the other 10 groups have 234 genes each). The horizontal red dashed line indicates the median of the second group. (D) As above, but displaying coding sequence lengths.