Fig. 2.

In situ hybridization analysis of VSIG1, ANXA10, and ACHE mRNAs in SSLs and cADNs. All three markers are strongly positive in SSLs (dense red punctate labeling in panels A, C, and E, reflecting “very high” expression (see scoring system depicted in Fig. 1) but absent in cADNs (panels B, D, and F) with the exception of few ANXA10- or ACHE-positive cells on their surface. While VSIG1 and ANXA10 are expressed in most of the longitudinal axis of the serrated glands, except their bottoms and tops, ACHE expression involves mainly the upper half of these crypts. Details on the expression patterns of these three mRNAs in the investigated lesions and in normal mucosa are shown in Supplementary Figs. 4, 5 and 6. Lesions are numbered as in Table 1