Fig. 5.

In situ hybridization analysis of MUC5AC and NKD1 mRNAs in SSLs and cADNs. MUC5AC is not a specific marker of SSLs as suggested by RNA-sequencing data: it is very highly expressed also in cADNs, although less extensively than in SSLs (i.e, patchy staining). NKD1 is very highly expressed in cADNs, but not in SSLs with the exception of a few cells at the bottom of their serrated glands. Details on the expression patterns of MUC5AC and NKD1 mRNAs in the investigated lesions and in normal mucosa are shown in Supplementary Figs. 13 and 14, respectively, and summarized in Table 1