Fig. 6.

In situ hybridization analysis of HOXD13, HOXB13, and FAM3A mRNAs in serrated lesions and cADNs. These three mRNAs are not specifically expressed in serrated lesions versus cADNs or vice versa. While HOXD13 and HOXB13 are expressed prevalently in lesions of the distal colon and rectum, FAM3A is prevalently found in proximal colon lesions: these patterns reflect the expression specificity in the normal mucosa in the distal and proximal segments of the large intestine. Details on the expression patterns of these three mRNAs in normal mucosa and in the investigated lesions are shown in Supplementary Figs. 15, 16, and 17, and summarized in Table 1