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TGFβ signalling is blunted by PIM1. (A) Representative western blot of pSMAD2 and total SMAD2 in GFP and PIM1 cells with and without treatment of 4 ng/mL TGFβ. (B) Quantification of pSMAD2 over total SMAD2. n = 5, error bars represent SEM, *P < 0.01 vs. GFP as measured by Student’s t-test. (C) Quantification of SERPINE1 and (D) N-CADHERIN mRNA expression via qRT–PCR. n = 3, error bars represent SEM, *P < 0.05 vs. GFP as measured by Student’s t-test.
