Figure 5.

Determination of mitochondrial and sarcomeric MMP-2 in the left ventricle of control and DXR mice by immuno transmission electron microscopy. (A and B) Representative localization of MMP-2 in the sarcomere (black arrows) and mitochondria (white arrows) from control and DXR hearts. MMP-2 is localized to the Z-disc (Z), M-line (M), I-band, and A-band region of the sarcomere. Scale bar = 500 nm. (C and D) Quantification of colloidal gold particles, conjugated to anti-MMP-2 antibody that were localized within the sarcomere and mitochondria. A total of 24 images were captured and analysed from 4 hearts from each group (n = 4). *P < 0.05 by paired two-tailed Student’s t-test.