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. 2021 Jan 6;14:83–95. doi: 10.2147/OTT.S277831

Figure 2.

Figure 2

Circ_0006404 interference inhibits the viability, metastasis and proliferation while induces the apoptosis of PCa cells. (A–G) LNCaP-AI and DU145 cells were transfected with si-NC or si-circ_0006404, and these transfected PCa cells were used to conduct functional experiments to assess the viability, metastasis, proliferation and apoptosis abilities. (A) Circ_0006404 level in PCa cells was examined by qRT-PCR. (B) The mRNA level of FOXO3 in PCa cells with circ_0006404 silencing or not was analyzed by qRT-PCR. (C) MTT assay was used to evaluate the cell viability of PCa cells. (D) Transwell assays were conducted to assess the influences of circ_0006404 interference on the migration and invasion of PCa cells. Magnification times: 100. (E) Colony formation assay was utilized to analyze the proliferation capacity of PCa cells transfected with si-NC or si-circ_0006404. (F) Cell cycle in PCa cells was assessed by flow cytometry. (G) Flow cytometry was used to assess the apoptosis rate of PCa cells in si-NC group and si-circ_0006404 group. *P<0.05.