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. 2021 Jan 6;14:83–95. doi: 10.2147/OTT.S277831

Figure 4.

Figure 4

Si-circ_0006404-mediated influences in PCa cells are partly weakened by the addition of miR-1299 inhibitor. (A–E) PCa cells were divided into four groups and transfected with si-NC, si-circ_0006404, si-circ_0006404 + inhibitor NC or si-circ_0006404 + miR-1299 inhibitor, respectively. (A) The viability of these transfected PCa cells was assessed by MTT assay. (B) The migration and invasion abilities of PCa cells were evaluated by transwell assays. (C) These transfected PCa cells were re-seeded into 6-well plates at a density of 200 cells/well. After 2-week cultivation, the visible colonies were counted to assess the influence of circ_0006404 and miR-1299 on the proliferation of PCa cells. (D) The effect of circ_0006404 and miR-1299 on the cell cycle of PCa cells was analyzed through measuring the percentages of cells in G0/G1 phase, S phase and G2/M phase via flow cytometry. (E) The apoptosis rate of PCa cells was measured by flow cytometry. *P<0.05.