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. 2021 Jan 6;14:1–13. doi: 10.2147/OTT.S280912

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© 2021 He et al.

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High-mobility group AT-hook 2 (HMGA2) is a direct target of miR-490-3p in osteosarcoma (OS) cells. (A and B) Cell Counting Kit 8 (CCK-8) assay and flow cytometric analysis were performed to investigate the proliferation and apoptosis of HOS and Saos-2 cells after miR-490-3p mimic or miR-NC injection. (C and D) Migration and invasion of miR-490-3p mimic-transfected or miR-NC-transfected HOS and Saos-2 cells were evaluated using Transwell migration and invasion assays. (E) Scheme showing the sequences of wild-type and mutant miR-490-3p binding sites in the 3′-untranslated region (UTR) of HMGA2. (F) Luciferase activity was detected in HOS and Saos-2 cells after cotransfection with miR-490-3p mimic or miR-NC and WT-HMGA2 or MUT-HMGA2. (G and H) HOS and Saos-2 cells transfected with miR-490-3p mimic or miR-NC were analyzed via reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting to quantify HMGA2 mRNA and protein levels. (I) mRNA expression levels HMGA2were detected in 57 pairs of OS tissues and adjacent normal tissues by RT-qPCR. (J) Inverse correlation between HMGA2 mRNA and miR-490-3p in the 57 OS tissues was verified by Pearson’s correlation coefficient. **P < 0.01.