Figure 10.

E coli–LF82–evoked release of cytosolic cytochrome C and mitochondrial fragmentation are reduced by gentamicin. T84 epithelia were treated with E coli–LF82 and examined for (A) the presence of cytosolic cytochrome C (∗P < .05 compared with ctrl, ^#P < .05 compared with 8 hours LF82; 1-way analysis of variance [ANOVA], Tukey multiple comparison test) or (B) pattern of the mitochondrial network 8 hours later. Time-matched epithelia were treated with gentamicin (200 μg/mL, 1 h) 4 hours after the addition of E coli and then examined 4 or 8 hours later (ie, the 8- and 12-hour gentamicin groups) (∗P < .05 compared with ctrl, ^#P < .05 compared with 8 h LF82 + gentamicin; 2-way ANOVA followed by the Tukey multiple comparison test; n = 5–12 epithelial preparation from 3–4 experiments; Ctrl, control; Sts, 20 μmol/L staurosporine). Data are means ± SEM.