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. 2020 Sep 28;11(2):551–571. doi: 10.1016/j.jcmgh.2020.09.013

Figure 4.

Figure 4

Adherent-invasive E coli–induced mitochondrial fragmentation is dependent on FimH attachment/invasion. (A) Representative confocal images of T84 mitochondrial networks stained with MitoTracker and (B and C) network quantification. ∗P < .05 compared with control, #P < .05 compared with HB101; 2-way analysis of variance (ANOVA) followed by the Tukey multiple comparison test, n = 5–6 epithelial monolayers (20 cells/monolayer) from 3 experiments. (D) Representative images and quantification showing that neither dead E coli–LF82 or spent medium (Med.) from E coli causes mitochondrial fragmentation in T84 epithelia. ∗P < .05 compared with control, 2-way ANOVA followed by the Tukey multiple comparison test, n = 8–9 epithelial preparations (20 cells/monolayer) from 3 experiments. (E) Bacteria lacking the FimH molecule are less invasive than the wild-type E coli–LF82 and fail to significantly induce fragmentation of the T84 epithelial cell mitochondrial network (representative images match the quantification bars). ∗P < .05 compared with control, 2-way ANOVA followed by the Tukey multiple comparison test, n = 6–18 epithelial monolayers from 5 experiments; 4 μmol/L CCCP. Data are means ± SEM. Ctrl, control; wt, wild type.