Figure 8.

E coli–LF82–induced decreased epithelial barrier function is reduced by co-treatment with an inhibitor of mitochondrial fission. (A) The E coli–induced (10⁸ CFU/mL) decrease in TER across filter-grown T84 monolayers was unaffected by co-treatment with Mdivi1 (5 μmol/L), P110 (10 μmol/L), or the pan-caspase inhibitor Z-VAD (100 μmol/L) (∗P < .05 compared with control [Ctrl], 2-way analysis of variance [ANOVA], Tukey comparison test, n = 12 epithelial preparations from 4 experiments; starting TER = 1000–2000 Ω × cm²). In contrast, (B) barrier assessment by 70 kilodalton dextran–fluorescein isothiocyanate (FITC) flux (∗P < .05 compared with ctrl, 2-way ANOVA, Tukey multiple comparison test, experiment number was considered as a separate variable rather than pooled data owing to large variations in fold change) and (C) transepithelial passage of the E coli were both reduced by Mdivi1 (t test). Data are means ± SEM.